Journal: mBio
Article Title: Impact of Paracoccin Gene Silencing on Paracoccidioides brasiliensis Virulence
doi: 10.1128/mBio.00537-17
Figure Lengend Snippet: Effect of PCN silencing on the morphological transition of P. brasiliensis cells. EV, As PCN1 , As PCN2 , and As PCN3 strains were grown in solid BHI medium or liquid DMEM at 25°C. The images represent the results of fluorescence microscopy of calcofluor white-stained cells from the various P. brasiliensis strains cultured for 1 and 10 days in DMEM. Bars correspond to 50 μm.
Article Snippet: P. brasiliensis strain 18 (WT) and PCN -downregulated strains (As PCN ) were maintained in brain heart infusion (BHI) solid or liquid media (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 1% glucose and ampicillin (100 μg/ml) or Dulbecco’s modified Eagle’s medium (DMEM) (Sigma-Aldrich) at 37°C on a mechanical shaker (200 rpm).
Techniques: Fluorescence, Microscopy, Staining, Cell Culture